@article{HGNHB8_2015_v34n1_52,
author={Sung-Jin. Lim and Jin-Hyo. Kim and Geun-Hyoung. Choi and Byung-Jun. Park},
title={Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography},
journal={Korean Journal of Environmental Agriculture},
issn={1225-3537},
year={2015},
volume={34},
number={1},
pages={52-56},
doi={10.5338/KJEA.2015.34.1.05},
url={http://dx.doi.org/10.5338/KJEA.2015.34.1.05}
TY - JOUR
AU - Lim, Sung-Jin.
AU - Kim, Jin-Hyo.
AU - Choi, Geun-Hyoung.
AU - Park, Byung-Jun.
TI - Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography
T2 - Korean Journal of Environmental Agriculture
PY - 2015
VL - 34
IS - 1
PB - The Korean Society of Environmental Agriculture
SP - 52-56
SN - 1225-3537
AB - BACKGROUND:Three commercial biopesticides containing derris extract, which is permitted as a commercial biopesticide substances by the Environmentally-friendly Agriculture Promotion Act, have been marketed in Korea. But, the quantitative analytical method of active substances for crop protection in biopesticides containing derris extract has not known.METHODS AND RESULTS:Solid phase extraction (SPE) cartridge clean-up method for the quantitative analysis of rotenone and deguelin in biopesticides containing derris extract was developed and validated by ultra-performance liquid chromatography (UPLC). The clean-up method was established using hydrophilic lipophilic balance (HLB) SPE cartridges for the bioactive substances in biopesticides containing derris extract, and the eluate was analyzed to quantify the rotenone and deguelin by the UPLC. LOQ and recovery rates of rotenone and deguelin were 0.085 and 0.044 mg/L, 95.7 and 93.3%, respectively. The content of rotenone and deguelin in three biopesticides containing derris extract were analyzed by the developed method, the results showed 0.001-0.236 and <LOQ-0.258%, respectively.CONCLUSION:The developed method could be applicable to determine the active substances and to control the quality of commercial biopesticide containing derris extract.
KW - Biopesticide
KW - Deguelin
KW - Derris
KW - Rotenone
KW - UPLC
DO - 10.5338/KJEA.2015.34.1.05
UR - http://dx.doi.org/10.5338/KJEA.2015.34.1.05
ER -
Lim, S. J., Kim, J. H., Choi, G. H., & Park, B. J. (2015). Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography. Korean Journal of Environmental Agriculture, 34(1), 52-56.
Lim, SJ, Kim, JH, Choi, GH & Park, BJ 2015, “Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography”, Korean Journal of Environmental Agriculture, vol. 34, no. 1, pp. 52-56. Available from: doi:10.5338/KJEA.2015.34.1.05
Lim, Sung-Jin et al. “Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography.” Korean Journal of Environmental Agriculture 34.1 (2015): 52-56.
1. Lim SJ, Kim JH, Choi GH, Park BJ. Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography. Korean Journal of Environmental Agriculture [Internet]. 2015;34(1): 52-56. Available from: doi:10.5338/KJEA.2015.34.1.05.
Lim, Sung-Jin, Jin-Hyo Kim, Geun-Hyoung Choi and Byung-Jun Park. “Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography.” Korean Journal of Environmental Agriculture 34, no.1 (2015): 52-56. doi: 10.5338/KJEA.2015.34.1.05.